Lijst van primers gebruikt in de fylogenie en identificatie van schimmels
Binnen de mycologie wordt gebruikt gemaakt van verschillende markers voor de fylogenie, multi-locus-sequence-typing.
Sommige van deze markers kunnen direct gebruikt worden voor identificatie.
Welke markers gebruikt worden is afhankelijk van het 'verwachte' organisme. Bij een niet-definieerbare isolaat wordt in eerste instantie de ITS regio's van het nrRNA gebruikt.
Marker | Sequence | Direction |
5.8G | AAGGTGCGTTCAAAGATTCG | Forward |
5.8S-R | TCGATGAAGAACGCAGC | Reverse |
ACT1 | CTGGGAYGAYATGGARAAGAT | Forward |
Act1F | GATCTGGCACCACACCTTCT | Forward |
Act1R | GCAGGTCTGAACAAGGCTTC | Reverse |
ACT2 | GYTCRGCCAGGATCTTCAT | Reverse |
ACT-512F | ATGTGCAAGGCCGGTTTCGC | Forward |
ACT-783R | TACGAGTCCTTCTGGCCCAT | Reverse |
ACTbw | AAGCCACCGATCCAGACG | Reverse |
ACTfw | TCTTCGAGACCTTCAACGCC | Forward |
actin-CA14 | AACTGGGATGACATGGAGAAGATCTGGC | Forward |
actin-CA5R | GTGAACAATGGATGGACCAGATTCGTCG | Reverse |
Al47-EF3-1650-f(P6-F) | GGHGGHTGGAAGATGAAG | Forward |
Al47-EF3-2451-R1(P6-R) | TCRTAVSWGTTCTTGAACTT | Reverse |
Al50+51-EF3-2900-F(P7-F) | TCYGGWGGHTGGAAGATGAAG | Forward |
Al50+51-EF3-3300-R(P7-R) | YTTGGTCTTGACACCNTC | Reverse |
apio_bt2rev | CGTGGCTTTAGCCAAAAAC | Reverse |
boydii_bt2rev | GTTTGAACCAAAAACTTACCT | Reverse |
Bt2a | GGTAACCAAATCGGTGCTGCTTTC | Forward |
Bt2b | ACCCTCAGTGTAGTGACCCTTGGC | Reverse |
BtpullA | ACTCCCTGCGCATCCTCACCC | Forward |
BtpullB | CTCCCACCTGCGCATCAACAA | Forward |
Btub2Fd | GTBCACCTYCARACCGGYCARTG | Forward |
Btub4Rd | CCRGAYTGRCCRAARACRAAGTTGTC | Reverse |
CAL-228F | GAGTTCAAGGAGGCCTTCTCCC | Forward |
CAL-737R | CATCTTTCTGGCCATCATGG | Reverse |
Cauris_FKS1HS1Fwd | TGGTGCTCAGCATTTGAGTC | Forward |
Cauris_FKS1HS1Rvd | ATTGTTGTCATCCTGCGACA | Reverse |
CaurisERG11dFwd | TGGGTKGGYTCWGCTGTTG | Forward |
CaurisERG11dRvd | TTCWGCTGGYTCCATTGG | Reverse |
CaurisERG11Fwd | GTGCCCATCGTCTACAACCT | Forward |
CaurisERG11Rvd | TCTCCCACTCGATTTCTGCT | Reverse |
CaurisFKS1Fwd | ATGTCTTACGATAACAATC | Forward |
CaurisFKS1Rvd | TTAGAATGCCTTTGTAGTATAG | Reverse |
CaurisMFalphaFwd | GAGAAAAGAGACGCTGAAGCTGAG | Forward |
CaurisMFalphaRvd | TCAACCTTCGAGGTCAGCTTCA | Reverse |
CL1 | GARTWCAAGGAGGCCTTCTC | Forward |
CL2A | TTTTTGCATCATGAGTTGGAC | Reverse |
CL2F | GACAAGGAYGGYGATGGT | Forward |
CL2R | TTCTGCATCATGAGYTGSAC | Reverse |
CL2R2 | CTTCTCGCCRATSGASGTCAT | Reverse |
D1 | AACTTAAGCATATCAATAAGCGGAGGA | Forward |
D2 | GGTCCGTGTTTCAAGACGG | Reverse |
ERG11FwdSeq_pos694 | AACTTCGTCTTCCCCCACTT | Forward |
ERG11RvdSeq_pos750 | CTGAGCAGCGTCTCTCTTCC | Reverse |
fRPB2-11aR | GCRTGGATCTTRTCRTCSACC | Reverse |
fRPB2-5F | GAYGAYMGWGATCAYTTYGG | Forward |
fRPB2-5R | CCRAARTGATCWCKRTCRTC | Reverse |
fRPB2-7cF | ATGGGYAARCAAGCYATGGG | Forward |
fRPB2-7cR | CCCATRGCTTGYTTRCCCAT | Reverse |
gRpb1-A | GAKTGTCCKGGWCATTTTGG | Forward |
gRpb1-C | CNGCDATNTCRTTRTCCATRTA | Reverse |
ITS1 | TCCGTAGGTGAACCTGCGG | Forward |
ITS1N | TCCGTWGGTGAACCWG | Forward |
ITS2 | GCTGCGTTCTTCATCGATGC | Reverse |
ITS3 | GCATCGATGAAGAACGCAGC | Forward |
ITS4 | TCCTCCGCTTATTGATATGC | Reverse |
ITS5 | GGAAGTAAAAGTCGTAACAAGG | Forward |
LR0R | ACCCGCTGAACTTAAGC | Forward |
LR5 | TCCTGAGGGAAACTTCG | Reverse |
LR7 | TACTACCACCAAGATCT | Reverse |
NL1 | GCATATCAATAAGCGGAGGAAAAG | Forward |
NL2m | CTTGTGCGCTATCGGTCTC | Reverse |
NL3m | GAGACCGATAGCGCACAAG | Forward |
NL4 | GGTCCGTGTTTCAAGACGG | Reverse |
NL4m | GGTCCGTGTTTCAAGACGG | Reverse |
NS1 | GTAGTCATATGCTTGTCTC | Forward |
NS2 | GGCTGCTGGCACCAGACTTGC | Reverse |
NS24 | AAACCTTGTTACGACTTTTA | Reverse |
NS3 | GCAAGTCTGGTGCCAGCAGCC | Forward |
NS4 | CTTCCGTCAATTCCTTTAAG | Forward |
NS5 | AACTTAAAGGAATTGACGGAAG | Reverse |
NS6 | GCATCACAGACCTGTTATTGCCTC | Forward |
NS7 | GAGGCAATAACAGGTCTGTGATGC | Forward |
NS8 | TCCGCAGGTTCACCTACGGA | Reverse |
pks1-F | GARGCNYTNGARATG | Forward |
pks1-R | CCDGTDCCGTGCATYTC | Reverse |
Rpb1F | CATGTGAGCTGGTGTGTATGC | Forward |
Rpb1-Fa | CAYAARGARTCYATGATGGGWC | Forward |
Rpb1-G2R | GTCATYTGDGTDGCDGGYTCDC | Reverse |
Rpb1R | CGAGCTTGAACGTCAAATCA | Reverse |
Rpb2-11aR | GCRTGGATCTTRTCRTCSACC | Reverse |
Rpb2-5F2 | GGGGWGAYCAGAAGAAGGC | Forward |
Rpb2-6F | TGGGGKWTGGTYTGYCCTGC | Forward |
Rpb2-7cF | ATGGGYAARCAAGCYATGG | Forward |
Rpb2-7cR | CCCATRGCTTGYTTRCCCAT | Reverse |
Rpb2-7R | CCCATWGCYTGCTTMCCCAT | Reverse |
Sdehoogii1-F | TCAGCGCCCGAAAGGACGAC | Forward |
T1 | AACATGCGTGAGATTGTAAGT | Forward |
T10 | ACGATAGGTTCACCTCCAGAC | Forward |
T2 | TAGTGACCCTTGGCCCAGTTG | Reverse |
T22 | TCTGGATGTTGTTGGGAATCC | Reverse |
T222 | TCAATTTTCCCATTCTCAACTT | Reverse |
T3B | AGGTCGCGGGTTCGAATCC | Forward |
V9 | TGCGTTGATTACGTCCCTGC | Forward |
V9G | TTACGTCCCTGCCCTTTGTA | Forward |